Figure 4.

In vitro production of interleukin (IL)-6 induced by IL-17A, but not by lipopolysaccharide (LPS), is inhibited by acetylsalicylic acid (ASA) treatment. (a) Antigen (ovalbumin (OVA)) uptake by bone marrow dendritic cells (BMDCs) after stimulation with LPS and different doses of ASA. (b) Flow cytometric analysis of BMDC maturation. BMDCs were stimulated with LPS (10 ng ml⁻¹) and ASA and then stained with anti-CD11c, anti-MHCII, anti-CD11b, and anti-CD80 antibodies. (c) Level of IL-6 in the culture supernatants of BMDCs stimulated with LPS (10 ng ml⁻¹) and ASA. (d) Level of IL-6 in the culture supernatants of Raw 264.7 cells activated by LPS (10 ng ml⁻¹), rIL-17A (50 ng ml⁻¹) or ASA. FITC, fluorescein. *P<0.05. MFI, mean fluorescent intensity.