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. 2013 Jan 10;45(1):e3. doi: 10.1038/emm.2013.1

Figure 3.

Figure 3

Restoration of miR-636 by adenine nucleotide translocase 2 (ANT2) short hairpin RNA (shRNA) inhibits cell proliferation and upregulation of miR-636 by ANT2 shRNA suppresses colony formation. (a) Hep3B cells were transfected with scramble, ANT2 shRNA, negative or miR-636 inhibitor. Cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)) assays were performed 24 h after transfection. Three independent experiments were performed. Data were analyzed using the Student's t-test. (b) Hep3B cells were transfected with negative or miR-636 mimics. Cell viability (MTT) assays were performed 24, 48 and 72 h after transfection. Three independent experiments were performed. Data were analyzed using the Student's t-test. (c) Colony formation after transfection of Hep3B cells with negative or miR-636 mimics.