Table 1. Gene-specific primers used for RT-PCR.
Gene name | Accession no. | Sequence | |
---|---|---|---|
BAFF | NM_033622 | Forward | 5′-TGCTACTCGGCTGGCATCGC-3′ |
Reverse | 5′-GCGCGGGCTCCGTTTCTCAT-3′ | ||
β-Actin | NM_007393 | Forward | 5′-AGCCATGTACGTAGCCATCC-3′ |
Reverse | 5′-TCCCTCTCAGCTGTGGTGGTGAA-3′ | ||
Leptin | NM_008493 | Forward | 5′-GAGACCCCTGTGTCGGTTC-3′ |
Reverse | 5′-CTGCGTGTGTGAAATGTCATTG-3′ | ||
Adiponectin | NM_017370 | Forward | 5′-GTCTCAGCTGTCCTTCCCCT-3′ |
Reverse | 5′-CCCTGGCTTTATGCTCTTTGC-3′ | ||
MCP-1 | NM_011331.2 | Forward | 5′-TTAAAAACCTGGATCGGAACCAA-3′ |
Reverse | 5′-GCATTAGCTTCAGATTTACGGGT-3′ | ||
COX-2 | NM_011198 | Forward | 5′-TGAGCAACTATTCCAAACCAGC-3′ |
Reverse | 5′-GCACGTAGTCTTCGATCACTATC-3′ | ||
IL-6 | NM_031168 | Forward | 5′-AGTTGCCTTCTTGGGACTGAT-3′ |
Reverse | 5′-TCCACGATTTCCCAGAGAAC-3′ | ||
Haptoglobin | NM_017370 | Forward | 5′-GACGAGAAGCAATGGGTGAAC-3′ |
Reverse | 5′-GCATCCATAGAGCCACCGA-3′ | ||
BAFF-R | NM_028075.2 | Forward | 5′-GACCCTGGTGGGTCTAGTGA-3′ |
Reverse | 5′-GTAGGAGCTGAGGCATGAGG-3′ | ||
BCMA | NM_011608.1 | Forward | 5′-ATCTTCTTGGGGCTGACCTT-3′ |
Reverse | 5′-CTCACAGGTGCACTCTTCCA-3′ | ||
TACI | NM_021349.1 | Forward | 5′-CTACTGCACACTGGGGGTCT-3′ |
Reverse | 5′-CTCCTGAGTGGGAGAACTGC-3′ |
Real-time reverse transcription (RT)-PCR experiments used the above primers.