Figure 1.

Rhamnogalacturonan II (RG-II) activates dendritic cell (DC) phenotypes. Bone marrow-derived dendritic cells (BMDCs) were incubated with 200 ng ml⁻¹ lipopolysaccharide (LPS; positive control) or 0.5 mg ml⁻¹ RG-II for 24 h. Co-stimulatory molecules were then analyzed by two-color flow cytometry. Cells were gated on a CD11c-positive population. DCs were stained with phycoerythrin (PE)-conjugated anti-CD86, anti-CD40 anti-MHC class I, or anti-MHC class II antibodies. Bar charts represent the mean (s.e.m.) of the percentages of positive DCs expressing CD86, major histocompatibility complex (MHC) class I, MHC class II or CD40. * And ** indicate significant differences at P<0.05 and P<0.01, respectively, compared with unstimulated cells. Results are representative of three independent experiments.