Figure 2.

Activated protein C (APC) differentially affects MG63 differentiation depending on the presence of specific bisphosphonates. (a) Alizarin red staining performed in MG63 cells pretreated with APC (10 μg ml⁻¹) for 1 h and/or cotreated with bisphosphonates (alendronate (Aln) 50 μℳ, pamidronate (Pam) 50 μℳ and zoledronate (Zol) 50 μℳ) for 48 h. The values were normalized to equal viable cell number. (b) Alkaline phosphatase activity tested in media of MG63 cells pretreated with APC (10 μg ml⁻¹) for 1 h and/or cotreated with bisphosphonates (alendronate 50 μℳ, pamidronate 50 μℳ, zoledronate 50 μℳ and lovastatin 5 μℳ) for 48 h. (c) Alkaline phosphatase activity tested in media and cell lysate of MG63 cells cultured with APC (0.1, 1 and 10 μg ml⁻¹) for 48 h. *P<0.05; **P<0.01 versus control group.