Figure 6.

Endothelial protein C receptor (EPCR) is expressed by MG63 cells and upregulated by activated protein C (APC). (a) MG63 cells were treated with APC (1 and 10 μg ml⁻¹) for 25 h, and cells were immunostained with EPCR antibody, and photographed in fluorescence microscopy analysis. (b) MG63 cells were pretreated with APC (10 μg ml⁻¹) for 1 h and/or cotreated with alendronate (Aln; 12.5, 25, 50 and 100 μℳ for 24 h, and cell lysates were analyzed by western blotting using antibodies for EPCR. (c) MG63 cells were pretreated with 10 μg ml⁻¹ of EPCR blocking antibodies (RCR-252) and 10 μg ml⁻¹ of APC for 1 h, and cotreated with Aln (100 μℳ) and zoledronate (Zol; 100 μℳ) for 48 h. **P<0.01 versus control group.