Table 3.
Association between Lymph Node micrometastatic status and normalized VEGF RT-PCR quantification cycles (Cq levels) in LNs
| Normalized Variable | Statistic | Lymph Node micrometastatic status | |||
|---|---|---|---|---|---|
|
| |||||
| Negative | Positive | Overall | P-value | ||
|
| |||||
| VEGF-A | Mean (SD) /N | 3.4 (1.7) /148 | −0.7 (4.1) /41 | 2.5 (2.9) /189 | <.0001 |
| Median (Range) | 3.5 (−2.7,6.5) | 0.8 (−14.1, 3.7) | 2.9 (−14.1,6.5) | ||
|
| |||||
| VEGF-C | Mean (SD)/N | 5.1 (1.7)/148 | 2.3 (3.6) / 41 | 4.5 (2.5) /189 | <.0001 |
| Median (Range) | 5.3 (−0.5, 8.3) | 3.7 (−6.9,6.5) | 5 (−6.9,8.3) | ||
|
| |||||
| VEGF-D | Mean (SD)/N | 10.6 (1.8) /148 | 6.7(4.1)/41 | 9.7 (3) /189 | <.0001 |
| Median (Range) | 10.9 (5.9, 14.3) | 7.6 (−1.5, 13.2) | 10.5 (−1.5, 14.3) | ||
|
| |||||
| VEGF-Receptor 3 | Mean (SD)/N | 6.7 (1.5)/148 | 5.2 (2.8) / 41 | 6.4 (2) / 189 | <.0025 |
| Median (Range) | 6.8 (2.1,9.8) | 6.1 (−1.1, 10.3) | 6.6 (−1.1, 10.3) | ||
VEGF = Vascular Endothelial Growth Factor; SD = Standard Deviation; N = Sample Number
Note: Lower Cq values reflect higher mRNA expression levels