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. Author manuscript; available in PMC: 2013 Feb 28.
Published in final edited form as: Cytometry A. 2011 Feb 10;79(3):233–240. doi: 10.1002/cyto.a.21027

Figure 2.

Figure 2

Testing responsiveness of U937 Cells. A) U937 cells were stained with Fluo-4. Then cells were sampled with the FACScan for 120 s, 10 μM ATP was added, and sampling resumed. At 380 s the sample was vortexed for 10 s and then sampling resumed. Inset shows typical raw forward and side scatter data used as a gate for final plots. B) U937 cells sampled with FACScan only and vortexed for 10 s.