Table 2. Mutation profile of six cell lines and five primary MM samples with respect to adhesion and RTK signaling.

Gene	AMO1	U266	OPM2	MM1S	JJN3	L363	Patient 1	Patient 2	Patient 3	Patient 4	Patient 5
MMP15		x(homoz.)		x					x
MUC4					xx (<2)				x
COL10A1				x			x
SDK1					x		x
LRFN5		x		x			x
THBS1	x						x
SLAMF1							x
COL6A3			xx					x
VCAN							x
THSD7A							x
ALOX12B			x (<2)				x
HSD17B1								x
NCAM2							x
ADAM29		x		x
ADAMTS1	x
ADAMTSL1				x(homoz.)
BCAN	x		x
COL17A1				x
COL7A1				x
DCHS1		x		x
DCHS2				x
ITGA10		x		x
ODZ1				x
SIGLEC1				x
WHSC1				x
LAMB2				x
PCDHGA7		x		xx	x
ADAMTS19		x (<2) x
ADAMTS9	x
CAMTA1			x
ADAMTS2	x (<2)		x
COL12A1						x
COL16A1						x
HS6ST3		x		x
LAMA5			x	x
NRXN1						x
ODZ4						x
PCDH10	x
ADAMTS18			x
CDH11						x
CELSR2					x
GPC6	x			x
ITGB1		x
LAMB1						x
PCDH15					x(homoz.)
PCDH17					x
PCDH20	x
RELN		xx
SPOCK1			x
TLN2						x
TNC	x			x (<2)
TGFBR2							x
CHIA				x(homoz.)
NTRK1				x							x
EPHB2							x
EPHA8		x		x			x
NTRK2						x					xx
EGFR	x			x
ERBB3	x					x
IGF1R						x
TTN					x (<2)
HK3							x
STAM2							x
IKBKE							x
ADRBK1							x
AKAP1							x
TYK2				x
KRAS	x			x						x
NRAS						x
BRAF		x
MAP2K2	x						x

Abbreviations: MM, multiple myeloma; RTK, receptor tyrosine kinase; x, mutated; xx, mutated twice in the same sample; homoz., homozygous

Adhesion molecules, RTKs and some downstream molecules are listed that were mutated in at least 1 of the5 primary MM or in at least 1 cell line, plus at least 1 of the 38 published primary MM.⁵ All mutations mentioned (x, xx, homoz.) were damaging in at least two functional predictors, if not otherwise specified (<2=less than 2 predictors). Genes that belonged to the same signaling network according to the STRING database or manual literature search were highlighted in bold and italics (bold: medium confidence; italics: low confidence). The mutation details for the 38 primary MM⁵ are shown in Supplementary Table S12.