Table 3. Correlation of selected mutations in RTKs and adhesion molecules with gene expression, CN changes and copy neutral LOH.

Gene	GE	CN	LOH	CL	Predictors
(A)
IGF1R	—	+		L363	3
NTRK2	—	—		L363	3
NTRK1	—	+		MM1S	2
EGFR	—	—		AMO1	2
EGFR	↑	—		MM1S	2
ERBB3	—	+		AMO1	3
ERBB3	—	—		L363	3
(B)
SDK1	—	+		JJN3	3
LRFN5	—	—	X	MM1S	3
LRFN5	—	—		U266	3
DCHS2	—	+		OPM2	3
DCHS2	—	—		MM1S	3
SIGLEC1	—	+		U266	2
SIGLEC1	—	—		MM1S	3
PCDHGA7	—	—		JJN3	2
PCDH10	—	—		AMO1	3
THBS1	—	—		AMO1	2
BCAN	—	++	X	AMO1	3
BCAN	↓	++		OPM2	3
LAMB2	↓↓↓	—	X	MM1S	3
CAMTA1	—	—		OPM2	3
ADAMTS2	—	—		OPM2	3
ADAMTS2	—	—		AMO1	2
LAMA5	↓↓	+		OPM2	2
LAMA5	—	—		MM1S	3
TNC	↑↑	—		AMO1	3
ADAMTS19	↓	—		U266	3
COL12A1	—	—		L363	3
CDH11	—	—		L363	3
CELSR2	—	+		JJN3	3
ITGB1	↓↓	—	X	U266	3
PCDH20	↑	+		AMO1	3
RELN	↓↓↓	+		U266	3

Abbreviations: CL, cell line; CN, copy number; GE, gene expression; LOH, loss of heterozygosity; MM, multiple myeloma; RTK, receptor tyrosine kinase.

One arrow represents a twofold up- or downregulation, one and a half arrows a threefold up- or downregulation, two arrows a fourfold up- or downregulation, and three arrows a ninefold up- or downregulation in mRNA expression compared with the median mRNA expression of all six cell lines; ± represents a simple copy number gain (three copies) and ++ represents four copies of that gene. Copy neutral LOH or LOH that was accompanied by a chromosomal gain is depicted by x.

^aRTKs and adhesion molecules that were mutated in at least 1 of the 43 primary MM (including Chapman et al.⁵) and 1 cell line, that were ‘damaging' by at least 2 functional predictors and that were validated by Sanger sequencing. These conditions apply to all genes of Table A and B.