Figure 6.

The expression of acetylated α-tubulin remained low for 48 h in the II18 cell line, whereas it increased with time in the RERF-LC-KJ cells. (A), The expressions of acetylated α-tubulin and α-tubulin were analyzed with the Western blot method using proteins extracted from cells exposed to 3.2 nM (−8.5 logM) paclitaxel for 6, 12, 18, 24 and 48 h and untreated (0 h). (B), The ratio of the expression level of acetylated α-tubulin to the total α-tubulin was measured. Columns, the mean of value of three experiments; bar, SD. (C), Cellular localization of acetylated microtubules (green) in cells exposed to paclitaxel for 6 and 24 h and untreated cells. The cells were stained with acetylated α-tubulin antibody, microtubules (red) with α-tubulin antibody and nuclei (blue) with DAPI. Bar, 50 μm. (D), The ratio of the expression of acetylated α-tubulin to that of α-tubulin obtained with immunofluorescent staining was calculated from integrating each fluorescent intensity on a series of stacked images with a thickness of 1 μm. Columns, mean of value obtained from 3 microscopic fields; bar, SD; ^**p<0.01 relative to respective untreated (0 h) cells.