Table I.
Set of primers for PCR amplification and nucleotide sequencing of β-tubulin isotype.
| Primer | Sequence for PCR amplification | Primer | Sequence for sequencing reaction |
|---|---|---|---|
| Class I | |||
| Forward (88–108) | ATACATACCTTGAGGCGAGCA | Forward (687–706) | CTCTCCGTCCATCAGTTGGT |
| Reverse (1488–1507) | GACGGCTAAGGGAACTGAGA | Reverse (1017–1036) | ACAGGCAGCCATCATGTTCT |
| Class II | |||
| Forward (83–100) | CACCATGCGCGAGATCGT | Forward (702–721) | GCCCTGTATGACATCTGCTT |
| Reverse (1461–1480) | CCATGCTTGAGGACAACAGA | Reverse (1052–1070) | CTCGTCCACCTCCTTCATG |
| Class III | |||
| Forward (65–84) | TATGAGGGAGATCGTGCACA | Forward (644–661) | GGAGAACACGGATGAGAC |
| Reverse (1462–1482) | GGTTTAGACACTGCTGGCTTC | Reverse (944–962) | CATGTTCTTGGCATCGAAC |
| Class IVa | |||
| Forward (22–39) | TCTCCGCCGCATCTTCCA | Forward (683–703) | GGTGGAGAATACGGATGAGAC |
| Reverse (1472–1492) | ATCAAAGGTCAGAAGCCTCGA | Reverse (983–1004) | CATGTTCTTGGCATCGAACATC |
| Class IVb | |||
| Forward (50–71) | TCTGCTGCTGTTTGTCTACTTC | Forward (636–654) | AGTGGTGGAGCCCTACAAC |
| Reverse (1461–1480) | GTTCACACTGCTTCCCTGCT | Reverse (985–1005) | AGCCATCATGTTCTTGGCATC |