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RecU depletion in 8325-4recUi strain leads to increased susceptibility to UV damage. Cultures of control strain BCBHV008 and recU inducible mutant 8325-4recUi showing serial dilutions from 10-2 (left) to 10-5 (right). 10 μl spots were placed on TSA agar, containing or not IPTG, and irradiated with a UV dose of 4 J/m2/sec for 0, 10, 20, 30 and 60 seconds. Plates were then incubated overnight and the number of CFU’s was counted.
