Table 2.
Primers used in this study
| Primer name | Sequence (5’-3’) |
|---|---|
| recUp1 |
ATCGAGATCTATGTACTTCAGGTGCGT |
| recUp2 |
TAGACTTTTTAAAATTTCACCACACAAGTTTGGTAG |
| recUp3 |
ACTTGTGTGGTGAAATTTTAAAAAGTCTATAAC |
| recUp4 |
ATCGGGATCCCAATGTTTTGACGTTC |
| recUp5 |
TGGTGTATTGTGTCTTTCG |
| recUp6 |
TTCCCACCATTATTACCG |
| recUp7 |
ATCTGCATGCTTAATTATGTTGGC |
| recUp8 |
ATACCCGGGTGTGTGGTGAAATTTATG |
| recUp9 |
TATGCTCGAGTCATACGCGGTCC |
| spoIIIEp1 |
GCTGCGGTACCGTCATAGCTATTTTAGTAGTTG |
| spoIIIEp2 |
GCTGCGGTACCGGAGGCGCCGCAGGACACCTCGTCATTATTAAGATC |
| spoIIIEp3 |
TGAGGATCCGATGAAAAATTCCCGTCT |
| spoIIIEp4 |
TACTCCCCGGGTTACTTGTACAGCTCGTCC |
| spoIIIEp5 |
TACTCCCCGGGCGGTCCACAAAAAGGAAG |
| spoIIIEp6 | TGCATTCCATGGGACATGCTGATCTTTGAATTTTGAAATTG |
Underlined sequences correspond to the restriction site. Bold sequences correspond to the five codon linker.