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. 2013 Mar;81(3):974–983. doi: 10.1128/IAI.01218-12

Fig 4.

Fig 4

Effects of single, double, and triple deletions of spyCEP, scpA, and sse on MGAS5005 virulence, skin invasion, and neutrophil recruitment in subcutaneously infection of mice. (A and B) Survival rates of 10 mice subcutaneously infected with 1.0 × 108 CFU MGAS5005, 1.0 × 108 CFU ΔscpA mutant bacteria, 1.5 × 108 CFU ΔspyCEP mutant bacteria, 1.4 × 108 CFU Δsse mutant bacteria, 1.6 × 108 CFU ΔspyCEP Δsse mutant bacteria, 1.6 × 108 CFU ΔscpA Δsse mutant bacteria, 1.4 × 108 CFU ΔscpA ΔspyCEP mutant bacteria, or 1.4 × 108 CFU ΔscpA ΔspyCEP Δsse mutant bacteria.(C and D) Lesion sizes (C) and neutrophil recruitment (D) at 24 h after subcutaneous infection of mice in two independent experiments. In experiment 1 (solid circles), mice were infected with 9.1 × 107 CFU MGAS5005, 1.1 × 108 CFU ΔspyCEP mutant bacteria, 9.2 × 107 CFU ΔscpA mutant bacteria, 1.7 × 108 Δsse mutant bacteria, 1.2 × 108 CFU ΔspyCEP Δsse mutant bacteria, 1.1 × 108 CFU ΔscpA Δsse mutant bacteria, 1.2 × 108 CFU ΔscpA ΔspyCEP mutant bacteria, or 1.2 × 108 CFU ΔscpA ΔspyCEP Δsse mutant bacteria. In experiment 2, mice were infected with 9.3 × 107 CFU MGAS5005, 1.2 × 108 CFU ΔspyCEP mutant bacteria, 1.0 × 108 CFU ΔscpA mutant bacteria, 1.5 × 108 CFU Δsse mutant bacteria, 1.1 × 108 CFU ΔspyCEP Δsse mutant bacteria, 1.0 × 108 CFU ΔscpA Δsse mutant bacteria, 1.2 × 108 CFU ΔscpA ΔspyCEP mutant bacteria, or 1.3 × 108 CFU ΔscpA ΔspyCEP Δsse mutant bacteria. One-way ANOVA of the PMN data: not significant, all pairs among MGAS5005 and the ΔspyCEP, ΔscpA, and ΔspyCEP ΔscpA mutants and pairs among the strains carrying Δsse; significant, all of the other pairs. One-way ANOVA of the lesion data: not significant, the Δsse mutant versus the ΔscpA Δsse mutant, the ΔspyCEP ΔscpA Δsse mutant versus the ΔspyCEP Δsse mutant, MGAS5005 versus the ΔspyCEP mutant, MGAS5005 versus the ΔscpA mutant, MGAS5005 versus the ΔspyCEP ΔscpA mutant, the ΔspyCEP ΔscpA mutant versus the ΔspyCEP mutant, the ΔspyCEP ΔscpA mutant versus the ΔscpA mutant, and the ΔscpA mutant versus the ΔspyCEP mutant; significant, all of the other pairs.