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. 2013 Mar;81(3):753–763. doi: 10.1128/IAI.00875-12

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Fig 1 — One-dimensional gel of S. oralis rGAPDH(Δ166-183) and 2D gel of P. gingivalis client proteins by a pulldown assay. (A) Purified S. oralis rGAPDH (Δ166-183) (3 μg) was subjected to SDS-PAGE (5 to 15% gradient gel) and stained with CBB. Lanes: M, molecular mass standard proteins; 1, S. oralis ATCC 9811 rGAPDH(Δ166-183). (B) P. gingivalis client proteins purified by a pulldown assay (100 μg) were separated by 2D gel electrophoresis and stained with CBB. The spots were identified as TonB-dependent receptor protein (RagA4; spot 1), 4-hydroxybutyryl-CoA dehydratase (AbfD; spot 2), lysine-specific proteinase (Kgp; spot 3), arginine-specific proteinase B (RgpB; spot 4), NAD-dependent glutamate dehydrogenase (GDH; spot 5), glyceraldehyde-3-phosphate dehydrogenase (GAPDH; spots 6 and 8), and malate dehydrogenase (MDH; spot 7).