Fig 3.

Effects of P. gingivalis recombinant client proteins on coaggregation between P. gingivalis and S. oralis by turbidimetric assay. Aliquots of suspensions of P. gingivalis ATCC 33277 (5 × 10⁸ cells) and S. oralis ATCC 9811 (5 × 10⁸ cells) with various concentrations of P. gingivalis recombinant client proteins were mixed simultaneously. The progress of coaggregation was monitored by measurement of the decrease in A₅₅₀ at 37°C, and the −dA/dt was calculated continuously. Coaggregation activity was calculated by subtraction of the −dA/dt of P. gingivalis ATCC 33277 alone from the maximum −dA/dt of both bacteria. Inhibition was determined with the following formula: (coaggregation activity without protein − coaggregation activity with protein)/(coaggregation activity without protein) × 100%. Minus value indicated enhancement of coaggregation. Values represent means ± standard deviations (SD) from three replicates.