Table 1.
Oligonucleotide primers used in this study
| Primer | Primer sequencea (5′–3′) | Reference or source |
|---|---|---|
| SogapdhΔ166-183F | AACTGCTTGGCTCCAATGGCTAAAGCTCTTCAAGACCAAATGATCCTTGACGGACCACAC | This study |
| SogapdhΔ166-183R | GTGTGGTCCGTCAAGGATCATTTGGTCTTGAAGAGCTTTAGCCATTGGAGCCAAGCAGTT | This study |
| rRagA4 SphI-F | GCGGCATGCAAAAGAATGACGCTATTCTTCCTTTGC | This study |
| rRagA4 SacI-R | CGCGAGCTCTTAGAAAGAAATCTGAATACCACC | This study |
| rRgpB BamHI-F | GCGGGATCCAAAAAGAATTTTAGCAGGATCGTT | This study |
| rRgpB SalI-R | CGCGTCGACTTACTTCACTATAACCTTTTCTGT | This study |
| rAbfD BamHI-F | GCGGGATCCACTAGCGAACAGTACGTAGAAAGT | This study |
| rAbfD SalI-R | CGCGTCGACTTACTTATCGAGTGATTCGTCGAT | This study |
| rKgp-cd SphI-F | GCGGCATGCGATGTTTATACAGATCATGGCGAC | This study |
| rKgp-cd SalI-R | CGCGTCGACTCAACGGGAAGCTTCTGCCTTCTT | This study |
| rGDH BamHI-F | GCGGGATCCAAGACCCAAGAAATTATGACAATG | This study |
| rGDH SalI-R | CGCGTCGACTTAGCAAACGCCCTGAGCTACCAT | This study |
| rGAPDH BamHI-F | GCGGGATCCACGAAAGTAGGTATTAACGGCTTT | This study |
| rGAPDH SphI-R | CGCGCATGCTTATGCGTTTACCTTAGCCATGTA | This study |
| rMDH BamHI-F | GCGGGATCCAGCTACTTAACAGAAGAGAAATTG | This study |
| rMDH SphI-R | CGCGCATGCTTAGAGGTTCGGATTCACCGTCTT | This study |
| Real-time RT-PCR | ||
| ragA4f | CCAGAATAGAACCGTGAAGG | This study |
| ragA4r | AAGCGTGAAGTTGCCATC | This study |
| rgpBf | TCGTAGCATTCTCCTCTC | This study |
| rgpBr | TGTGAACTGAAGATTGTCC | This study |
| abfDf | CAACAAGACTTCCGCCATCC | This study |
| abfDr | AACGCAGAACACGCATACG | This study |
| kgp-cdf | CGGTTCGTATGCTTGTTGTTG | This study |
| kgp-cdr | GATAGAGGCGTTTGTCGTTCC | This study |
| gdhf | AAGCCAGCGAATACTATGTAGC | This study |
| gdhr | GCAGCCACTTGTCCACTTC | This study |
| gapdhf | GCAGCAGGCGGCAATATC | This study |
| gapdhr | GGCACACGGAATGACATACC | This study |
| mdhf | GCCTTGTTACGCTTATCTAC | This study |
| mdhr | TGATGCCGAAGTGCTTAG | This study |
| fimAf | TTGTTGGGACTTGCTGCTCTTG | 29 |
| fimAr | TTCGGCTGATTTGATGGCTTCC | 29 |
| luxSf | GAATGAAAGAGCCCAATCG | 29 |
| luxSr | GTAATCGCCTCGCATCAG | 29 |
| 16Sf | AGGAACTCCGATTGCGAAGG | 29 |
| 16Sr | TCGTTTACTGCGTGGACTACC | 29 |
a
Underlined sequences represent restriction enzyme sites.