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Validation of microarray data by quantitative RT-PCR of plasmid-borne and eight chromosomal genes, including glgA, CTL0071, CTL0305, CTL0339, CTL0397 to CTL0399, and CTL0638, significantly (P < 0.01) differentially expressed in L2-versus-L2R and L2Rp+-versus-L2pΔpgp4 comparisons. Transcript copy numbers derived from three replicates were normalized to those of lpdA mRNA.
