Fig 8.

Mapping of surface-exposed immunogenic regions using mouse anti-PE peptide Abs. (A) Ribbon diagram showing PE amino acid regions (indicated with different colors) that were selected for immunization of a series of mice. (B) Surface structure of selected regions as shown in panel A. (C) Results from ELISA that demonstrate Ab recognition of peptides with which microtiter plates were coated. (D) Recognition of full-length recombinant PE 22 to 160 by peptide Abs shown in ELISA. (E) Flow cytometry profiles of NTHI showing surface recognition of PE at the surfaces of bacteria using anti-PE peptide Abs.