Fig 5.

Activation of TLR4 in HEK 293 cells transfected with human TLR4-MD2-CD14 (A) and TLR2 in HEK 293 cells stably transfected with human TLR2-CD14 (B). TLR activation was monitored colorimetrically using a SEAP reporter gene placed under the control of an NF-κB inducible promoter. Tenfold serial dilutions of whole bacterial cells of the indicated strains of C. jejuni were added to each well in triplicate. Values represent the means and standard deviations of one experiment assayed in triplicate. The graphs are representative of three independent experiments. Asterisks indicate a P value of <0.005 compared to wild-type 81-176 or the complement. Black lines, wild-type 81-176; red lines, the isogenic kpsM mutant; blue lines, the kpsM mutant complemented in trans.