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Graphic diagrams of the characteristics of four different CSPs. Full-length CSP consists of the signal peptide (SP), two highly conserved motifs at the N- and C-terminal ends of the protein, region 1 and region 2 plus (R1 and R2), tandem repeats (37 NANP repeats), and the GPI anchor signal (GPI). CSP1 and CSP2 were produced by expression in Escherichia coli, CSP3 and CSP4 by expression in P. pastoris.
