Fig 2.

C57BL/6 mice (n = 4) were immunized twice, 3 weeks apart, with either 2 μg or 20 μg of various CSPs and 50 μg poly(I·C)LC. Naive mice and mice that received poly(I·C)LC alone or 20 μg of each protein alone served as controls. Four weeks later, T cells in spleens were analyzed for cytokine production by flow cytometry. Frequencies of CSP-specific IFN-γ-, IL-2-, TNF-α-, or IL-10-producing CD4⁺ T cells are shown for protein-only and control mice (A) and mice that received either 2 μg (B) or 20 μg (C) of CSP with poly(I·C)LC. Data are expressed as means + SD and are representative of two independent experiments. *, P < 0.05. The color of the asterisk indicates the group to which the value is being compared.