Fig 8.
C57BL/6 mice (n = 10 per group) were immunized three times, 3 weeks apart, with 20 μg of CSP1 together with 50 μg poly(I·C)LC as the adjuvant. Mice that received poly(I·C)LC or naive mice served as controls. (A) Five weeks later, mice were challenged i.v. with 1 × 103 Pb-CS(Pf) sporozoites. Starting on day 4, daily blood smears were taken and analyzed under a microscope. Kaplan-Meier plots show the time to detection of parasites in the blood for each vaccine group after the challenge. (B) Sera collected 3 weeks before challenge were analyzed for CSP-specific IgG antibodies. Endpoint titers are grouped by whether mice went on to be protected from challenge. *, P < 0.05. Results are representative of two independent experiments.