Fig 1.

Three FPI genes with unknown contributions to F. tularensis Schu S4 virulence. (A) Schematic of iglI, iglJ, and pdpC genes within the FPI. The site of insertion is marked with a triangle (nt 608). (B) PCR amplification across the target region (the primers are marked by bars above and below the arrow in panel A) shows group II intron-mediated insertions (915 bp) into both copies of pdpC (lane 1) compared to the DNA control without insertions (lane 2). Lane M, molecular weight marker. (C) Primers were designed to amplify nucleotides 81 to 599 (pdpCfrag) or 1503 to 1749 (pdpCfull) of pdpC or a region of pdpE. Each primer set was used for RT-PCR of the wild type or a pdpC mutant. −RT, no-reverse transcriptase controls.