Fig 6.

Different roles of PdpC, IglI, and IglJ in phagosome escape. Phagosome integrity was quantified 90 min after infection of human MDMs with GFP-expressing bacteria using digitonin to selectively permeabilize the plasma membrane and introduce anti-F. tularensis antibodies into the MDM cytosol. Background staining was determined using formalin-killed (FK) Schu S4 and by omission of digitonin. The data are the means ± SD from three independent experiments. **, P < 0.01 versus Schu S4; ++, P < 0.01 versus FK Schu S4.