Fig 7.

Colocalization of all three mutants with LAMP-1. MDMs were infected in parallel with Schu S4, fevR, iglI, iglI⁺, iglJ, iglJ⁺, pdpC, or pdpC⁺ organisms. At 7.5 h postinfection, samples were processed for confocal microscopy and double stained to detect bacteria (red) and LAMP-1 (green). (A) Representative images. The arrows indicate bacteria that colocalize with LAMP-1. (B) Pooled data are the means and SD from multiple independent experiments (n ≥ 4). **, P < 0.01 versus Schu S4.