Table 1.
Relative affinities of toxT promoter mutants for membranes containing (ToxR+) or lacking (ToxR−) ToxR
| Targeta | Amt (mg/ml) of protein required for a 50% shift |
Fold increase relative to wild type | |
|---|---|---|---|
| ToxR+ | ToxR− | ||
| Wild type (−172 to +45) | 0.76 | > 5.6 | 1.0 |
| T(−100)G | 0.57 | > 5.6 | 0.8 |
| A(−99)C | 0.96 | > 5.6 | 1.3 |
| T(−98)G | 0.74 | > 4.2 | 1.0 |
| C(−97)A | 0.99 | > 5.6 | 1.3 |
| T(−96)G | 1.55 | > 5.6 | 2.0 |
| C(−-95)A | 0.44 | > 5.6 | 0.6 |
| A(−94)C | 2.01 | > 5.6 | 2.6 |
| A(−93)C | 2.33 | > 5.6 | 3.1 |
| A(−92)C | 2.02 | > 5.6 | 2.7 |
| A(−91)C | 2.12 | > 5.6 | 2.8 |
| A(−90)C | 1.83 | > 5.6 | 2.4 |
| A(−89)C | 1.48 | > 5.6 | 1.9 |
| C(−88)A | 0.77 | > 4.2 | 1.0 |
| A(−87)C | 0.74 | > 5.6 | 1.0 |
| T(−86)G | 3.42 | > 5.6 | 4.5 |
| A(−85)C | 1.23 | > 5.6 | 1.6 |
| A(−84)C | 4.12 | > 5.6 | 5.4 |
| A(−83)C | 1.98 | > 5.6 | 2.6 |
| A(−82)C | 1.39 | > 5.6 | 1.8 |
| T(−81)G | 0.67 | > 4.2 | 0.9 |
| A(−80)C | 0.74 | > 4.2 | 1.0 |
| T(−60)G | 0.83 | > 5.6 | 1.1 |
| A(−84)T | 2.39 | > 4.2 | 3.1 |
| toxT −100 to +45 | 0.74 | > 5.6 | 1.0 |
a
Numbers represent the position of the promoter mutation or the endpoints of deletions, relative to the toxT transcription start site. Bold nucleotides indicate the position of the ToxR-binding site direct repeat TNAAA-N5-TNAAA (−96 to −82).