Fig 1.
Nicotinic acid treatment triggers growth inhibition and premature motility. E-phase cultures of WT L. pneumophila carrying the flaA-GFP gene reporter were supplemented with water (H2O) or 5 mM nicotinic acid (NA). At the times indicated, samples of each culture were collected to quantify bacterial growth as determined by optical density (A) and expression of the transmission gene flaA by relative fluorescence (B). Shown are representative graphs from three or more independent experiments. At 6 and 9 h posttreatment, the optical densities and fluorescence levels were statistically different between E-phase cultures treated with water and E-phase bacteria treated with 5 mM NA (P < 0.05).