Table 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype/phenotype | Reference or source |
|---|---|---|
| E. coli strains | ||
| DH5α | F− endA1 hsdR17 (r− m+) supE44 thi-l recA1 gryA (Nal1) relA1 Δ(lacZYA-argF−)U169 ϕ80dlacZΔM15 λpirRK6 | Laboratory Collection |
| DY330 | W3110 ΔlacU169 gal490 λcI857 Δ(cro-bioA) | 77 |
| MB739 | HB101 endA::frt | 36 |
| MB741 | DH5α pMMBGent | 36 |
| MB776 | HB101 λpir endA::frt pR6KcatrdxArpsL | 36 |
| MB870 | DH5α pGEMlpg0272-3 | This work |
| MB871 | DH5α pGEMlpg0272::FRT-cat-FRT | This work |
| MB872 | HB101 endA::frt pGEMlpg0273::cat-rdxA-rpsL | This work |
| MB873 | DH5α pMMBGent-lpg0273 | This work |
| L. pneumophila strains | Wild type; thyA hsdR rpsL | 35 |
| MB110 | ||
| MB355 | MB110 pflaG | 8 |
| MB414 | letA::kan pflaG | 10 |
| MB417 | letS::kan pflaG | 10 |
| MB860 | Lp02 pMMBGent | This work |
| MB861 | Lp02 pMMBGent-lpg0273 | This work |
| MB862 | Lp02 Δlpg0273 pMMBGent | This work |
| MB863 | Lp02 Δlpg0273 pMMBGent-lpg0273 | This work |
| MB864 | Lp02 Δlpg0272::FRT-cat-FRT | This work |
| MB865 | Lp02 Δlpg0272::FRT | This work |
| MB866 | Lp02 lpg0273::cat-rdxA-rpsL | This work |
| MB867 | Lp02 Δlpg0273 | This work |
| Plasmids | ||
| pGEM-T easy | Multiple cloning site within coding region of β-lactamase α fragment linearized with single-T overhangs, 3 kb; Ampr | Promega |
| pflaG | 150-bp flaA promoter fragment fused to GFP gene, encodes thymidylate synthetase, 10.5 kb; Ampr | 8 |
| pKD3 | FRT-cat-FRT allele | 78 |
| pMMBGent | Broad-host-range plasmid pMMB67EH encoding gentamicin resistance | 8 |