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In vitro release of rSAG1 from PLG microparticles over a 35-day period. A total of 5 mg of PLG-rSAG1 microparticles was suspended in 1 ml of PBS (pH 7.4) with 0.02% sodium azide and shaken at 37°C in 1.5 ml microfuge tubes for 35 days. The amount of rSAG1 in the supernatant sampled daily was measured using the BCA protein assay. The release studies were carried out in triplicate, with each point representing the mean ± SD.
