Fig. 4.

Effects of 5-LOX, 12-LOX, COX-1 and COX-2 knockdown on DHA-induced apoptosis in PC3 cells. PC3 cells were transfected with control siRNA, 5-LOX siRNA (A), 12-LOX siRNA(B), COX-1 siRNA (C) or COX-2 siRNA (D) for 6h and then supplemented with growth medium containing 1% FBS overnight. Cells were treated with DHA-BSA (30 µM) for 48h. Caspase-3 activity was measured with the Caspase-Glo® 3/7 assay. Values expressed relative to controls representing the mean ± SD (n = 3) with different letters are significantly different (P < 0.05).