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. 2012 Oct 12;34(1):176–182. doi: 10.1093/carcin/bgs324

Fig. 5.

Fig. 5.

Effects of 15-LOX-1 and 15-LOX-2 knockdown on SDC-1 upregulation by DHA in PC3 cells. A and B. PC3 were transfected with control siRNA, 15-LOX-1 siRNA (A) or 15-LOX-2 siRNA (B) for 6h and then supplemented with growth medium containing 1% FBS overnight. Cells were treated with DHA-BSA (30 µM) for 8h. Total RNA from cells was isolated, and SDC-1 mRNA was measured by RT-PCR. Values compared with relevant control with different letters are significantly different (P < 0.05). C and D. PC3 were transfected with control siRNA, 15-LOX-1 siRNA (C) or 15-LOX-2 siRNA (D) for 6h and then supplemented with growth medium containing 1% FBS overnight. Cells were treated with DHA-BSA (30 µM) for 48h. Protein extracts were used for western blot analysis of SDC-1 and β-actin. Bar graphs are scans of SDC-1 corrected for β-actin and shown relative to control. Data shown are representative of three independent experiments. Relative ratios are shown in graphs, and values representing the mean ± SE (n = 3) with different letters are significantly different (P < 0.05).