Fig. 6.

15-LOX-1 knockdown blocks the effect of DHA on phosphorylations of PDK1 and Akt. PC3 cells were transfected with control siRNA, 15-LOX-1 siRNA or 15-LOX-2 siRNA for 6h and then supplemented with growth medium containing 1% FBS overnight. Cells were treated with DHA (30 µM) for 48h before harvest. PDK1, p-PDK1 (S243), total Akt and pAkt (T308 and S473) were measured by western blot assay. Data shown are representative of three independent experiments. Relative ratios are shown in the graph, and values representing the mean ± SE (n = 3) with different letters are significantly different (P < 0.05)