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Cross-linking of CaM or GSH-CaM to the ryanodine receptor (RyR2). (A) Representative immunoblot of CaM or GSH-CaM cross-linked to RyR2. Note that the anti-CaM (or GSH-CaM) antibody detected only RyR2 among the many proteins in the SR. (B) Effect of the antibody against the CaM-binding domain (3583–3603; anti-CaMBD antibody) of RyR2 on CaM (or GSH-CaM) binding to RyR2.
