Figure 1. Comparing in vitro reconstituted CENP-A nucleosomes to CENP-A nucleosome purified from human cells using AFM. (A) AFM micrographs showing in vitro reconstituted bulk nucleosomes onto plasmids containing α-satellite DNA derived from human centromeres (left), in vitro reconstituted chromatin digested for 1 min with MNase (middle), and IP’ed in vitro reconstituted CENP-A chromatin digested with MNase (right). Scale bar = 10 nm. (B) Graphical representation of AFM measurements comparing in vitro (listed above and Table 1) and in vivo samples from Bui et al., 2012. Dotted red line designates nucleosomes of mean octameric heights.