Table 1. Conditions of FRAP protocols used in experiments in nonreplicating nuclei, replication foci and DNA repair sites.

	Nonreplicating nuclei	Replication foci	DNA repair sites	Parameter
GFP-labeled proteins (free GFP, HP1β, PCNA)	2.36 Hz	4.83 Hz	4.88 Hz	Pre and post bleach scanning frequency
	244 μW of 488 nm			Imaging laser power
	Square 3 μm × 3 μm	1 μm diameter circle		ROI
	For 1.2 sec 4.6 mW of 488 nm + 1.6 mW of 476 nm	For 10 ms 1.8 mW of 488 nm + 600 μW of 476 nm	For 10 ms 120 μW of 488 nm	Bleaching insult
YFP-labeled HP1-PCNA complex	2.36 Hz	0.483 Hz	4.88 Hz (recovery) 0.49 Hz (plateau)	Pre and post bleach scanning frequency
	680 μW of 514 nm			Imaging laser power
	Square 3 μm × 3 μm	1 μm diameter circle		ROI
	For 1.2 sec 0.95 mW of 496 nm + 4.65 mW of 514 nm	For 10 ms 1.8 mW of 514 nm	For 10 ms 210 μW of 514 nm	Bleaching insult