Table 3. Summary of mass spectrometry parameters for each species.
Species of Interest | SRM Transition (m/z) | Internal Standard Transition (m/z) | Qualifier Ion Transition (m/z) | Fragmentor Voltage (V) | Collision Energy (V) |
Lysine | 147→130 | 151→134 | NA | 100 | 8 |
Formyllysine | 175→112 | 179→116 | NA | 105 | 10 |
Acetyllysine | 189→126 | 197→134 | NA | 105 | 10 |
Mono-methyllysine | 161→84 | NA | 161→130 | 100 | 10 |
Di-methyllysine | 175→84 | NA | 175→130 | 100 | 14 |
Tri-methyllysine | 189→84 | NA | 189→130 | 100 | 14 |