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Effect of 5-hydroxylation on N-acetylation of ϵ-amino group of lysyl residues
Lineweaver-Burk plots were used for estimation of the kinetic constants, Vmax, and Km. R2 is the determination coefficient (see Fig. 6C).
| Vmax | Km | |
|---|---|---|
| μm/min | μm | |
| H41–23 + p300 | 35.34 ± 1.65 (R2 = 0.9981) | 204.51 ± 10.12 |
| H41–23 OH + p300 | 6.95 ± 0.45 (R2 = 0.9902) | 200.37 ± 14.32 |
