FIGURE 6.

Structural models of linker mutations in open and closed states. A, the structural models of T4840W in the open and closed states depict relative stabilization due to the mutation in the open state caused by formation of new van der Waals contacts. Residues forming contacts with residue 4840 are shown with surface representation, with the largest such interaction observed for T4840W in the open state. B, the structural models of H4832W in the open and closed states depict destabilization of the open state due to clashes between residue 4832 and residues in the S5 helix from the adjacent monomer (shown as red dotted lines) and no such clashes in the closed state. Both mutants are compared with WT.