TABLE 3.
Properties of 45L mutants
The values denote the mean ± S.E. NA, not applicable.
| RyR1 | Cells with caffeine response | Bmax of [3H]ryanodine bindinga | Intensity on immunoblots | γb | PCa/PKc |
|---|---|---|---|---|---|
| % WT | % WT | % WT | pS | ||
| WT | NA | NA | NA | 802 ± 8 (12) | 6.6 ± 0.1 (18) |
| V4830W | 22.5 ± 4.3 (20)d | 20.4 ± 11.1 (6)d | 51.5 ± 6.3 (14)d | 786 ± 6 (10) | 6.7 ± 0.2 (5) |
| H4832W | 67.2 ± 12.0 (9)d | 79.4 ± 13.5 (4) | 70.3 ± 6.2 (8)d | 785 ± 7 (14) | 6.4 ± 0.2 (5) |
| G4834W | 85.0 ± 10.5 (8) | 49.0 ± 14.5 (3)d | 123.6 ± 17.1 (7) | 791 ± 6 (15) | 6.6 ± 0.2 (6) |
| V4838W | 81.1 ± 10.9 (7) | 58.5 ± 11.4 (3)d | 104.5 ± 17.6 (13) | 772 ± 4 (17) | 6.8 ± 0.3 (4) |
| T4840W | 127.0 ± 8.5 (13)d | 30.0 ± 12.3 (3)d | 71.8 ± 8.9 (10)d | 770 ± 6 (7) | 6.7 ± 0.1 (5) |
| V4841W | 95.1 ± 5.3 (7) | 43.2 ± 13.3 (4)d | 63 ± 8.3 (8)d | 783 ± 7 (13) | 6.3 ± 0.2 (5) |
a Maximum binding capacity.
b K+ conductance.
c Selectivity of Ca2+ over K+.
d p < 0.05 compared with WT.