FIGURE 7.

Ovol2 is up-regulated by BMP4 and inhibits neural specification in the chick embryo. A, ISH analysis of cOvol2 (a–e), cSox3 (f–h), and cSox2 (i and j) in HH stage 1–5 chick embryos. B, double ISH for cOvol2 (blue) and cSox2/3 (magenta) in HH stage 3+ to 5 chick embryo. C, the expression of cOvol2 (blue) in the chick embryos electroporated with control GFP or BMP4-IRES-GFP plasmid was examined by ISH (a and c), and subsequent immunostaining for GFP (brown) marked the electroporated cells in the same embryos (b and d). D, the expression of cSox2 (blue) in the chick embryos electroporated with control GFP or cOvol2-IRES-GFP plasmid was examined as described in C. The electroporated field is highlighted by broken lines.