FIGURE 3.
Logo diagrams of flanking extein sequences for 483 randomly chosen positive hits selected for splicing of the Npu DnaE intein in KanR site C were made using the Weblogo program (28). The vertical red line indicates the position of the intein. The +1 position was fixed as Cys. A, shown is the entire library of 483 clones. B, shown are all clones with Trp+2. C, shown are all clones with Met+2. D, shown are all clones with Gly at −3 or −2. E, shown are all clones with Trp+2 and a hydrophilic residue at +3. F, shown is all clones with Trp+2 and a hydrophobic residue at +3.