TABLE 3.

Kinetics of trans-splicing by the Npu DnaE intein with the listed flanking extein sequences in M-I^N and I^C-P^His

Kinetic parameters were measured as described under “Experimental Procedures” for selection hits (E2, E4, E6, E12, and E16), the native extein, the EP clone selected from a low stringency pilot experiment with the native RBS, and an inactive intein with mutations in essential residues C1A in M-I^N and the intein C-terminal Asn plus C+1A in I^C-P^His.

* No trans-splicing product was detected after 1 hour.