TABLE 1.

T_m values determined using CD

	WT	R1597W (Tm)	M1528V	E1638K
		°C
1.25 mm Ca	67.7 ± 0.1	53.9 ± 0.1	57.9 ± 0.1	50.2 ± 0.1
1 mm EDTA	56.7 ± 0.1	51.5 ± 0.1	42.6 ± 0.2