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Analysis of the PIC on the HIS4 promoter by psoralen cross-linking of DNA
| Analyzed molecules | Molecules with a bubble | Bubble size (mean ± S.E.; nucleotides) | |
|---|---|---|---|
| No NTP (with fixation)a | 57 | 25 (43.8%) | 67.6 ± 2.62 |
| +ATP, GTP, and CTP (with fixation) | 85 | 10 (11.8%) | 78.5 ± 5.0 |
| DNA alone | 100 | 11 (11.0%) | 65.5 ± 5.34 |
a The PIC was fixed with glutaraldehyde.
