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Effects of pH and divalent cations on App1p PAP activity. A, PAP activity was measured at the indicated pH values with 50 mm Tris-maleate-glycine buffer. B, PAP activity was measured in the absence and presence of the indicated concentrations of MgCl2 or MnCl2. C, PAP activity was measured with 1 mm MgCl2 in the absence and presence of the indicated concentrations of CaCl2, MnCl2, or ZnCl2. The data shown are means ± S.D. (error bars) from triplicate enzyme determinations.
