FIGURE 4.

Effects of temperature on App1p PAP activity and stability. A, PAP activity was measured at the indicated temperatures for 20 min in a temperature-controlled water bath. B, the enzyme samples were first incubated for 20 min at the indicated temperatures. After incubation, the samples were cooled in an ice bath for 10 min to allow for enzyme renaturation, and PAP activity was then measured for 20 min at 30 °C. The data shown are means ± S.D. (error bars) from triplicate enzyme determinations.