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Time dependence of the App1p PAP reaction. PAP activity was measured with 2 mm [32P]PA (500 cpm/nmol) and 10 ng enzyme for the indicated time intervals. Following the incubations, the water-soluble 32Pi hydrolyzed from the substrate was analyzed by scintillation counting. The data shown are means ± S.D. (error bars) from triplicate enzyme determinations.
