FIGURE 1.
MMP23-PD suppresses KV1.3 but not KV1.2 currents. A, schematic of full-length MMP23 (MMP23-FL) showing its functional domains. B, schematic of DsRed-tagged MMP23-FL and C-terminal deletion constructs. CatDom, catalytic domain. C, KV1.3 and KV1.2 currents in the presence or absence of MMP23-FL (red) and MMP23-PD (green). Scale bars represent 1 nA and 50 ms, respectively. D, scattergrams showing channel numbers/cell of KV1.3 and KV1.2 channels in the presence or absence of MMP23-FL or deletion constructs. Means are determined from n samples of 8–38 for KV1.3 and 6–17 for KV1.2, respectively. Error bars indicate S.E. The membrane capacitances of the patched cells were 15.1 ± 1.0 pF (cells co-expressing the DsRed vector), 15.4 ± 1.1 pF (cells co-expressing DsRed-MMP23-FL), and 18.36 ± 1.6 pF (cells co-expressing DsRed-MMP23-PD). Statistical significance is determined by Student's t test and indicated by p values.